Chameleon® 800 Pre-stained Protein Ladder

Components

928-80000

Chameleon 800 Pre-stained Protein Ladder, 500 μL

Specifications

  • Channel: 800 nm
  • Size: 500 μL
  • Storage: Store in small aliquots at -15 to -25 °C and always protect from light. Do not subject the ladder to more than 25 cycles of freezing and thawing.
  • Shelf Life: 6 months from date of receipt
  • Compatible Imagers Include: Odyssey M Imager, Odyssey DLx Imager, Odyssey CLx Imager, Odyssey XF Imager, Odyssey Fc Imager

Loading Volumes

Application Recommended Loading Volume
Protein Gel 10 μL
Western Blot 1 - 5 μL*

*Ladder volume may require optimization to obtain uniform signal compared to your sample.

Product Description

The Chameleon 800 Pre-stained Protein Ladder provides multicolored, pre-stained, and near-infrared fluorescent bands of convenient and consistent protein sizes.

In gels, the ladder can be used to visualize progress of the protein separation during electrophoresis and to estimate the molecular weight of unknown proteins based on their relative mobility. In blotting applications, the ladder can be used to monitor protein transfer and as a reference to estimate the molecular weight of proteins of interest.

The example shows a visible image (left) and an 800 nm channel, near-infrared image (right) resolved on a 4 - 12% Bis-Tris Gel and transferred to nitrocellulose via wet tank transfer. The blot was scanned on an Odyssey CLx Imager.

The ladder is optimized for use with Bis-Tris and Tris-Glycine systems. To see a complete portfolio of LI-COR molecular weight markers, visit licorbio.com/proteinmarkers.

 Chameleon Duo Marker band sizes 800

Protocol

Chameleon Pre-stained Protein Ladders are provided ready to use in loading buffer. Bring the product to room temperature while protecting from light. Mix the product thoroughly before use to dissolve any solids that may have precipitated during storage. Do not heat above room temperature.

During electrophoresis, the ladder will separate into 6 visible and near-infrared bands in the 8 - 260 kDa range. The ladder can be used for easy visualization or near-infrared detection in the 800 nm channel. For individual molecular weights, please refer to the image above.

The Chameleon Pre-stained Protein Ladders are not standards, and protein band migration may appear different depending on the gel type used.

Analysis

Empiria Studio® Software

Molecular weights for Chameleon Pre-Stained Protein ladders are preset in Empiria Studio Software, allowing you to estimate band size without entering the molecular weights. For more information, see the instructions for the Experiment workflow you are using at https://www.licorbio.com/support/answer-portal/software/empiria-studio.html.

Image Studio™ Software

For instructions on entering marker molecular weights and sizing bands in Image Studio Software, visit https://www.licorbio.com/chameleon-sizing. Although Image Studio Software is no longer in development, we continue to sell and support it.