Intercept® T20 Protein-Free Antibody Diluent

Intercept T20 (TBS) Protein-Free Antibody Diluent

P/N

Description

927-85001

500 mL

927-85003

3 x 500 mL

927-85010

10 x 500 mL

Intercept T20 (PBS) Protein-Free Antibody Diluent

P/N

Description

927-95001

500 mL

927-95003

3 x 500 mL

927-95010

10 x 500 mL

Specifications

The Intercept Protein-Free Blocking Buffer in the antibody diluent now uses an eco-friendly ingredient. While this ingredient changes the appearance of the diluent, the diluent still performs the same. Check the certificate of analysis to see if your diluent uses this ingredient.

Shake well before each use.

  • Shelf Life: See expiration date on bottle.

  • Storage: 4 °C

  • Contains a 0.05% concentration of Tween® 20.

    Because of the high purity and stability of the Tween 20 used in Intercept T20 Protein-Free Antibody Diluent, the concentration of 0.05% is sufficient. When using standard research grade Tween 20, use a concentration of 0.2%. See the NIR Western Blot Protocol (licorbio.com/wbanalysis) for more information.

  • Suitable for a variety of applications, including: quantitative Western blots, chemiluminescent Western blots, In‑Cell Western™ Assay, In-Gel Western, protein array, and glycoprotein detection.

Description

Intercept T20 Antibody Diluents improve specificity of primary and secondary antibodies, reducing off-target effects. The diluent contains Intercept (PBS) Protein-Free Blocking Buffer or Intercept (TBS) Protein-Free Blocking Buffer preformulated with Tween® 20. This diluent can be used where animal-based products are prohibited.

Since there’s no need to mix the diluent yourself, you can save time and reduce steps in your protocol that might lead to variability in your results.

Intercept T20 Protein-Free Antibody Diluent is ideal for use with Intercept Protein-Free Blocking Buffer.

Protocol

Shake well before each use.

For a detailed Western blot protocol, see the Near-Infrared (NIR) Western Blot Detection Protocol (licorbio.com/wbanalysis). For troubleshooting ideas, see Good Westerns Gone Bad: Tips to Make Your NIR Western Blot Great (licorbio.com/GWGBIR).

Preparing Secondary Antibody Diluent

If you're using a PVDF membrane, add SDS to a final concentration of 0.01 - 0.02% in Intercept Protein-Free Antibody Diluent when preparing the secondary antibody diluent.

See the Near-Infrared Western Blot Protocol (licorbio.com/wbanalysis) for more detail.

Related Products

See licorbio.com/intercept for ordering information.

Blocking Buffer

  • Intercept (PBS) Blocking Buffer
  • Intercept (TBS) Blocking Buffer
  • Intercept (PBS) Protein-Free Blocking Buffer
  • Intercept (TBS) Protein-Free Blocking Buffer

Antibody Diluent

  • Intercept T20 (PBS) Antibody Diluent
  • Intercept T20 (TBS) Antibody Diluent