VRDye™ 490 Goat anti-Rabbit IgG Secondary Antibody

Applications

VRDye 490 Secondary Antibodies can be used for a variety of applications, including Western blotting. Visit the LI-COR® website (licorbio.com) for more information.

VRDye 490 Goat (polyclonal) anti-Rabbit IgG (H+L) (P/N 926-49020) is suitable for multiplex labeling experiments when combined with other secondary antibodies labeled with proper fluorescent dyes and when using instrumentation with appropriate excitation and detection capabilities. For example, two- or three-color detection can be achieved by multiplexing VRDye 490 Secondary Antibodies with IRDye® 680RD or IRDye 680LT and IRDye 800CW secondary antibodies for detection on the Odyssey M Imager or Odyssey F Imager.

Specifications

  • Quantity: 0.5 mg, lyophilized

  • Antibody Concentration: 1.0 mg/mL when reconstituted as directed

  • Fluorophore: VRDye 490 (1011 g/mole)

  • Excitation Wavelength: 492 nm (in PBS)

  • Emission Wavelength: 522 nm (in PBS)

  • Immunogen: Rabbit IgG

  • Form of Antibody: VRDye 490 conjugated purified immunoglobulin, lyophilized in phosphate buffered saline, pH 7.4. Contains 10 mg/mL Bovine serum albumin (BSA) (IgG and protease free) as stabilizer and 0.01% sodium azide as preservative after reconstitution.

    Contains sodium azide.

Storage and Reconstitution

Lyophilized

After Reconstitution
  • Storage temperature: 4 °C
  • Storage conditions: Protect from light.
  • Shelf life: This product is stable for six months when stored as recommended.
  • Storage temperature: 4 °C
  • Storage conditions: Protect from light.
  • Shelf life: Expiration date is listed on the product's label.

When ready for use, prepare a 1.0 mg/mL stock solution by reconstituting contents of vial with 0.5 mL sterile distilled water. Mix gently by inverting. Allow to rehydrate for at least 30 minutes before use. Centrifuge product if solution is not completely transparent after standing at room temperature. Immediately dilute stock solution as recommended for each specific application prior to use. Do not re-use product.

Suggested Dilution Ranges

Suggested working dilutions are given as a guide only. Optimum dilutions will vary and should be determined empirically.

Application Recommended Suggested Range
Western blot detection 1:10,000 1:5,000 – 1:15,000
Microscopy 1:400 1:100 – 1:1,000
Flow cytometry 1:1,000 1:200 – 1:2,000
Other User Optimized  

Purity and Specificity

Isolation of specific antibodies was accomplished by affinity chromatography using pooled IgG covalently linked to agarose. Based on ELISA and flow cytometry, this antibody reacts with heavy and light chains of rabbit IgG and light chains of rabbit IgM and IgA. This antibody was tested by dot blot and/or solid phase adsorbed for minimal cross-reactivity with human, mouse, rat, sheep, and chicken serum proteins but may cross-react with immunoglobulins from other species. The conjugate has been specifically tested and qualified for immunofluorescence microscopy, flow cytometry, and Western blot applications.