Applications

VRDye 549 Streptavidin (P/N 926-54030) can be used as a secondary detection reagent for microscopy, protein arrays, In-Gel Westerns, and Western blotting. Centrifuge briefly before use to eliminate aggregates that may have formed in solution. This will reduce non-specific background staining.

Properties

• Quantity: 0.5 mg

• Storage: 4 °C protected from light. Expiration date is listed on the product's label.

• Physical State: Liquid

• Concentration: 1 mg/ml

• Fluorophore: VRDye 549 (1040 g/mole)

• Excitation Wavelength: 555 nm

• Emission Wavelength: 569 nm

• Buffer: 10 mM Phosphate, 183 mM NaCl, and 2.7 mM KCl, pH 7.4 with 0.005% (w/v) sodium azide as a preservative.

  Contains sodium azide.

Suggested Dilution Ranges

Suggested working dilutions are given as a guide only. Optimum dilutions will vary and should be determined empirically.

For membrane-based applications and In-Gel Westerns, it is recommended to add SDS (0.02% to 0.1% final concentration) and Tween® 20 (0.1 to 0.2% final concentration) during the detection incubation step to reduce non-specific background staining.