WesternSure® HRP Goat anti-Mouse IgG (H + L)
Components (926-80010)
HRP-conjugated Goat anti-Mouse IgG (H + L) (adsorbed against human immunoglobulins)
Specifications
- Size: 1.0 mL (liquid)
- Source: Pooled antisera from goats hyperimmunized with mouse IgG paraproteins
- Form of Antibody: HRP-conjugated purified immunoglobulin, supplied in 50% glycerol / 50% PBS, pH 7.4. No preservative added.
- Appearance: Light brown
- Storage: 4 °C
- Shelf Life: Product is stable for 12 months at 4 °C
Suggested Dilution Ranges
| Substrate | WesternSure HRP Goat anti-Mouse IgG |
| WesternSure Chemiluminescent Substrates | 1:5,000 - 1:100,000 |
| Other | User optimized |
Purity and Specificity
The antibody was purified by affinity chromatography on pooled mouse IgG covalently linked to agarose. Based on ELISA and / or flow cytometry, the antibody reacts with the heavy and light chains of mouse IgG1, IgG2a, IgG2b, and IgG3, along with the light chains of mouse IgM and IgA. The antibody has minimal cross-reactivity with human immunoglobulins.
Applications
WesternSure HRP-conjugated secondary antibodies can be used for detection of chemiluminescent Western blots and are optimized for use with the LI‑COR C‑DiGit Blot Scanner and the Odyssey Fc Imaging System. WesternSure HRP conjugates are compatible with a variety of chemiluminescent substrates but are optimized for use with WesternSure PREMIUM Chemiluminescent Substrates. The sensitivity, intensity, and duration of the signal allow for easy detection using imaging systems or film.