Qualitative Western Blot Overview

Qualitative Western Blot Experiments provide quick signal identification data and charts.

Qualitative Western Blot Experiments are similar to Quantitative Western Blot Experiments, but Quantitative Western Blot Experiments include additional validation Experiments and provide additional calculations that you may not need for quick signal identification.

Summary of Differences between Qualitative and Quantitative Western Blot Experiments

This table highlights some key differences between Qualitative and Quantitative Western Blot Experiments.

	Qualitative Western Blot	Quantitative Western Blot
Antibody Validation
Linear Range Determination
Housekeeping Protein Validation
Warnings on Target Analysis Starting Pages for Incomplete Validation Experiments
Set up calculations step in Target Analysis (used to setup fold change calculations, etc.)

Overview of Qualitative Western Blot Experiments

Experiment Type	Purpose	Process	Detection	Maximum Images	Maximum Targets
Antibody Validation	Validate primary antibodies for use in your intended assay and sample context.	Evaluate antibodies in your assay to confirm expected performance.	Fluorescence, chemiluminescence	3	4
Analysis: TPS + Targets	Compare target bands to total protein.	Quantify and compare the total protein and target signals in the chosen blot images.	Fluorescence	3	4
Qualitative Analysis: Target Proteins	Compare target protein bands.	Quantify and compare the target protein band signals in your chosen image(s).	Fluorescence, chemiluminescence	3	4