Recommended Dilutions for Western blotting
Product | Recommended | Suggested Range |
IRDye® 800CW | 1:20,000 | 1:5,000 – 1:25,000 |
IRDye® 680RD | 1:20,000 | 1:5,000 – 1:25,000 |
IRDye® 680LT | 1:20,000 | 1:10,000 – 1:50,000 |
VRDye™ 549 | 1:10,000 | 1:5,000 – 1:15,000 |
VRDye™ 490 | 1:10,000 | 1:5,000 – 1:15,000 |