Recommended Dilutions for Western blotting

Product Recommended Suggested Range
IRDye® 800CW 1:20,000 1:5,000 – 1:25,000
IRDye® 680RD 1:20,000 1:5,000 – 1:25,000
IRDye® 680LT 1:20,000 1:10,000 – 1:50,000
VRDye™ 549 1:10,000 1:5,000 – 1:15,000
VRDye™ 490 1:10,000 1:5,000 – 1:15,000