Receptor-based Binding and Competition Cell-Based Assays with Infrared Fluorescence

Thursday, 5 September, 2013


  • receptor binding assay
  • cell-based assay
  • on-cell western assay
  • internalization assay

    • What is an On-Cell Western? It is a cell-based assay that enables quantitative monitoring of cell surface protein expression. The On-Cell Western assay offers the ability to:

    • Detect and quantify target proteins localized to the cell surface
    • Quantify ligand binding to cell surface receptors
    • Monitor receptor internalization and recycling by detecting loss and re-appearance at the cell surface
    • Perform and detect cell surface biotinylation assays
    • Evaluate the effects of mutations, drugs, and other treatments on protein trafficking
    • Analyze many samples quickly and quantitatively
    • Avoid use of radioactivity

    Visit the On-Cell Westerns application page for more information. 

    There is also a sample protocol of On-Cell Western Assay for Targeted Near-infrared-labeled optical imaging agent development. 

    For other scientific publications in which On-Cell Westerns were used, visit our publications database.

    Figure 1. Cannabinoid receptor 1 (CB1) is internalized after exposure to a specific agonist (Win-2), but the effect is blocked by the antagonist SR1. A) Intensity levels were greatly reduced in wells treated with 1μM of the CB1-specific agonist, Win-2. Cells treated with Win-2 and the specific CB1 antagonist, SR1 displayed no reduction of signal with the treatment. B) Graph displaying results of three independent experiments done in quadruplicate. Reprinted with permission from Miller, J.W. Tracking G protein-coupled receptor trafficking using Odyssey imaging.LI-COR Biosciences application note (2004).

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